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However, the trial had limitations: it neither measured ferroptosis biomarkers (e.g., ACSL4/Ptgs2) nor included monotherapy control arms, thereby precluding validation of the putative anti-ferroptotic mechanism or synergistic superiority of the combination

In animal models, BPC-157 has demonstrated remarkable properties across multiple organ systems: Tissue repair: Accelerates healing of muscles, tendons, ligaments, bones, and skin (Sikiric et al., 2018) Gastrointestinal protection: Protects and heals the gut lining, reduces intestinal inflammation, and counteracts NSAID-induced damage (Sikiric et al., 2016) Anti-inflammatory effects: Reduces systemic inflammation and modulates inflammatory cytokine expression Angiogenesis: Promotes new blood vessel formation, improving blood flow to injured tissues (Hsieh et al., 2017) Neuroprotection: Shows protective effects on the central and peripheral nervous system in animal models Nitric oxide modulation: Interacts with the nitric oxide (NO) system to support vascular health and tissue perfusion The peptide is sometimes called a healing peptide because of its broad tissue-protective properties

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Biochemistry 43:89748986

Eur J Pharmacol 332:2333 Sikiric P, Marovic A, Matoz W et al (1999) A behavioural study of the effect of pentadecapeptide BPC 157 in Parkinsons disease models in mice and gastric lesions induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydrophyridine

To confirm the binding mode between c-MET II and HGF I as shown in our structure, we introduced three point mutations in the K1 domain of HGF, including E159R, E195R, and R197E, to disrupt this interface, and examined the effects of these mutations on c-MET activation